The 2,4-Dimethyl-3-pentanone (DIPK) is a promising candidate biofuel for automotive applications and is produced by the fungal conversion process that can be optimized for widespread of biofuel utilization. There are few studies in the literature on combustion properties of DIPK, such as, ignition delay times and laminar burning velocity (LBV) measurements. However, all studies are conducted at low pressures (near 1 atm) and are far away from the conditions present inside automotive applications that work at higher pressures. Therefore, we present LBV measurements at high initial pressures up to 10 atm. It is known that the flame in a constant volume bomb develops cellular structure (hydrodynamic instability) as the initial pressure increases because of the reduction in flame thickness and the increase of density jump along one isentrope. In addition, the diffusional-thermal instability prevents experiments for rich mixtures because of the reduction of Lewis number. An earlier study from our lab showed that the flame instability prevented a proper extraction of LBV for stoichiometric and rich mixtures at 5 atm if O2 and N2 are used as diluent. In this study, Helium (He), and Argon (Ar) are used to suppress flame instability at 5 atm. Several oxygen to diluent ratios are used at 5 atm, 130 °C, and a wide range of equivalence ratio (0.7-1.5) to provide the general trend of LBV. It is noticed that the new diluent suppressed the flame instability, in the case of (He), a smooth spherical flame without cellular structure can be obtained even at an equivalence ratio of 1.5. Since the constant volume approach is used for determining LBV, many data points can be extracted out of a single experiment (up to 10 atm and 225 °C) which delivers several validation points for chemical kinetic mechanisms that are developed for DIPK.