Optimal trailer design for tractor-trailer transportation depends on accurate and reliable estimates of typical lifetime loading duty cycles. On-the-road testing was done to measure the vertical acceleration response of three different trailers in forest logging conditions. The test conditions included various trailer axle and load configurations, weight transported, and road surfaces including both gravel and paved roads. It was concluded that the rigid trailer body bounce and pitch, the non-rigid body trailer bending, and the wheel hop are the primary components of the vertical response. These results establish approximately what types of operational amplitudes are to be expected for these operations and will provide the basis for the development of a more general trailer frame load prediction model.