A better understanding of turbulent kinetic energy is important for improvement of fuel-air mixing, which can lead to lower emissions and reduced fuel consumption. An in-cylinder flow study was conducted using 1548 Laser Doppler Velocimetry (LDV) measurements inside one cylinder of a 3.5L four-valve engine. The measurement method, which simultaneously collects three-dimensional velocity data through a quartz cylinder, allowed a volumetric evaluation of turbulent kinetic energy (TKE) inside an automotive engine. The results were animated on a UNIX workstation, using a 3D wireframe model. The data visualization software allowed the computation of TKE isosurfaces, and identified regions of higher turbulence within the cylinder. The mean velocity fields created complex flow patterns with symmetries about the center plane between the two intake valves. High levels of TKE were found in regions of high shear flow, attributed to the collisions of intake flows. During the compression stroke the higher TKE was located around the momentary center of the cylinder volume. The TKE inside the cylinder decayed exponentially with time.