Advanced Approach to Simultaneous Monitoring of Multiple Bacteria in Space 972422
A novel microarray-based approach to simultaneous detection, quantitation, and identification of multiple microorganisms found in water and air supplies is presented. The rapid DNA/RNA probe-based approach avoids time consuming cell cultivation procedures by utilizing the inherent amplification property of rRNA combined with highly sensitive proximal CCD detection.
Specifically, optimal DNA probes for simultaneously capturing Escherichia coli and Vibrio proteolyticus were arrayed in a 4 x 4 matrix using a novel microarray printer capable of depositing hundreds of probes within a single well (1cm2) of a conventional 96 well microtiter plate. Following hybridization of a 16S rRNA microorganism mixture to the DNA microarray, detection, imaging, quantitation and identification was performed within seconds employing the complimentary PC-based proximal CCD detector/imager.
Citation: Eggers, M., Balch, W., Mendoza, L., Gangadharan, R. et al., "Advanced Approach to Simultaneous Monitoring of Multiple Bacteria in Space," SAE Technical Paper 972422, 1997, https://doi.org/10.4271/972422. Download Citation
Author(s):
Mitchell D. Eggers, William J. Balch, Leopoldo G. Mendoza, Ramprasad Gangadharan, Arnab K. Mallik, Michael G. McMahon, Michael E. Hogan, Du Xaio, Thomas R. Powdrill, Bonnie Iverson, George E. Fox, Richard C. Willson, Karine I. Maillard, Janet L. Siefert, Narinder Singh
Affiliated:
Genometrix Inc., Baylor College of Medicine, University of Houston
Pages: 10
Event:
International Conference On Environmental Systems
ISSN:
0148-7191
e-ISSN:
2688-3627
Also in:
SAE 1997 Transactions - Journal of Aerospace-V106-1
Related Topics:
Identification
Bacteria
Imaging and visualization
Water
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