In order to clarify the characteristics of turbulence in diesel combustion, fluctuations of pressure and temperature were measured in a DI diesel engine, and the root mean square value, the auto-correlation coefficient and the power spectrum density of the measured fluctuations were analyzed comparing between the cases with and without pilot injection. The following concluding remarks are obtained. (1) The frequency power spectrum of in-cylinder pressure history is decreased by pilot injection in two frequency ranges from 0.2 to 2.0 kHz and from 2 to 5 kHz. (2) Fluctuation of combustion pressure is dependent on the maximum rate of pressure rise, which is dependent on ignition delay. (3) The maximum RMS of soot temperature fluctuation in the diffusion combustion is dependent on the maximum RMS of pressure fluctuation in the initial combustion. (4) Fluctuation of temperature during diffusion combustion period has the characteristics of isotropic turbulence.