Single-laser-shot measurements of the fuel/air ratio in the cylinder of a motored direct-injection natural gas (DING) engine were obtained using a dual-pump coherent anti-Stokes Raman scattering (CARS) technique capable of simultaneously probing N2 and CH4. The DING engine was modified for optical access and CARS was used to probe the region near the glow plug. Measurements were acquired at eight different probe volume locations with one crank angle degree resolution for injections starting at 30° and 20° BTDC. The CARS data clearly show the arrival of the fuel jet at the probe volume and, from traversing the probe volume, the location of the centerlines of two fuel jets in the vicinity of the glow plug. The CARS measurements also show large fluctuations in fuel concentration on a shot-to-shot basis indicating the presence of large-scale mixing structures within the fuel jets.