An energy balance was performed on the life support equipment used during the Phase III, 90-day, human Lunar-Mars Life Support Test Project at the Johnson Space Center. The purpose of the analysis was to account for all the energy sources, uses, and losses in the test-bed. Knowledge from this task may allow more energy efficient designs to be developed. Control volumes were defined and energy balance equations were generated for major systems. The analyses succeeded in balancing the energy fairly well for several systems. Further, the data showed that inefficiencies existed, and means of design optimization were subsequently suggested.